Microscopy
Microscopy is one of the Garvan Institute’s major
capabilities. The Garvan has a dedicated Microscopy Facility located in
the basement of The Kinghorn Cancer Centre as well as various
microscopes throughout the Garvan and Kinghorn buildings to provide
researchers and visiting scientists with easy access to a huge variety
of state-of-the-art imaging techniques for single-molecules, organelles,
cells, tissues, intra-vital and whole animal imaging.
The principal microscopes include:
- Leica DM 6000 Power Mosaic: Advanced transmitted light and
widefield fluorescence microscope with high speed stepping stage and
focus for fast scanning and mosaic image acquisition
- Leica DMI 6000 : Advanced widefield fluorescence microscope for
live cells also with lasers for total internal reflection fluorescence
imaging and autofocus
- Leica DMI 6000 SP8 : Advanced laser scanning confocal microscope
- Leica DMI 6000 SP8 SMD MP: Advanced confocal and multiphoton
fluorescence microscope for live cells and tissues with high speed (12K)
resonant- and field-of-view- scanners and equipment for fluorescence
lifetime imaging
These and a number of our other instruments are capable of
imaging tissue, cells or intracellular organelles and molecules using a
variety of transmitted light (brightfield, phase contrast, interference
contrast) and fluorescence-based techniques; such as epifluorescence,
laser scanning confocal and total internal reflection fluorescence
microscopy (TIRF-M, ‘evanescent wave microscopy’). Two of our
microscopes are permanently set up with incubation equipment to allow
live cell time-lapse microscopy for short (hours) or long (days) term
experiments using any of our available imaging techniques even at high
speeds.
In addition, we have equipment to perform experiments using
techniques such as Förster Resonant Energy Transfer (FRET),
fluorescence recovery after photobleaching (FRAP), colocalization
analysis by either confocal, fluorescence or TIRF microscopy.
For more information, please click here
For further information please contact Dr William Hughes at w.hughes@garvan.org.au.